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KMID : 0613820130230060721
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Journal of Life Science
2013 Volume.23 No. 6 p.721 ~ p.727
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Analysis of Genetic Relationship of Apple Varieties using Microsatellite Markers
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Hong Jee-Hwa
Kwon Yong-Sham
Choi Keun-Jin
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Abstract
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The objective of this study was to evaluate the suitability of microsatellite markers for variety identification in 42 apple varieties. For microsatellite analysis, 305 primer pairs were screened in 8 varieties and twenty six primer pairs showed polymorphism with clear band pattern and repetitive reproducibility. A total of 165 polymorphic amplified fragments were obtained in 42 varieties using 26 markers. Two to twelve alleles were detected for each locus with an average of 6.4 alleles per locus. A value of polymorphism information content (PIC) ranged from 0.461 to 0.849 with an average of 0.665. A total of 165 marker loci were used to calculate Jaccard's distance coefficients using unweighted pair-group method with arithmetical average (UPGMA) cluster analysis. Genetic distance of cluster ranged from 0.27 to 1.00. Analysis of genetic relationship revealed that these 26 microsatellite marker sets discriminated a total of 41 varieties except for 1 variety among 42 varieties. These markers will be utilized as molecular data in variety identification of apple.
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KEYWORD
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Apple, genetic relationship, genotype, microsatellite marker, variety identification
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